HEK293 cells stably expressing CB1 Cannabinoid Receptor, cAMP biosensor & β-arrestin biosensor
Description
MPX NOMAD Cells are cell lines stably co-expressing tag-free GPCRs and two Nomad Biosensors to monitor G-protein and β-arrestin modulation. Each vial of MPX NOMAD CB1 Cannabinoid Receptor Cell Line contains HEK293 cells stably expressing the following constructs:
human α1B-adrenoceptor with no tag
cAMP Nomad Biosensor (Red Fluorescence)
β-arrestin Nomad Biosensor (Green Fluorescence)
MPX NOMAD CB1 Cannabinoid Receptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; G protein by cAMP flux and β-arrestin recruitment, hence allowing biased activity studies. When an agonist binds to CB1 a G protein is activated which, in turn, triggers a cellular response mediated by cAMP and a subsequent internalization mediated by ß-Arrestin. This leads to an increase of fluorescence intensity of both β-Arrestin and cAMP biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
20 tests available in this pack
Référence interne:
SA-CAS9-68
URL de site web:
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